In this experiment, the aqueous bitter kola extract was added to the plasma invitro.
The glucose concentration gotten was then compared with that of the normal blood glucose without the extract.
Test
Six tubes were lined and 100*l of plasma was put into each tubes. Into first three tubes was labelled 20*lE, 50*lE, 100*lE of extract. The second set of three test tubes was labelled 20*l NS,
50*l NS,100*lNS, of normal saline which acted as control.
In test tubes labelled for extract 20*l, 50*l and 100*l of the bitter kola extract was put respectively. The same was done to the normal saline. The tubes were shacked for content to mix. The timing device was set to 1 hour.
This allows a time for the extract to react with the blood glucose. At the end of one hour a blood glucose test was done (as described above). The values were calculated according. The time device set to another one hour, making the reaction time to be two hours. At the end of the two hours the blood glucose test were repeated and values calculated accordingly.